96-Plex library prep kit

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Title:  

96-Plex library prep kit

Agency:  

U.S. Department Of Agriculture

State:  

Maryland

NAICS Code:  

541714

Solicitation Type:  

Request for Quotation

Solicitation ID:  

12405B25R0010

Open Date:  

7/15/2025

Close Date:  

7/25/2025

Last Updated:  

7/20/2025

Description:
The United States Department of Agriculture (USDA) is seeking a contractor to provide at least 8640 individual plant sample sequencing library kits. The kits must contain all reagents needed to prepare double-stranded libraries with full-length adapters compatible with Illumina sequencing chemistries from 50 nanograms of high molecular weight plant DNA. The kits should include unique DNA sequence barcodes for at least 960 samples that allow samples to be pooled for Illumina whole genome sequencing and later deconvoluted to associate DNA sequences with individual samples. • The kits must be able to prepare libraries in four hours or less. • The kits must not require equipment beyond pipettes/tips, a vortex mixer, a benchtop mini-centrifuge, 96-well thermal cycler with heated lid, a fluorometer, and Agilent Technologies 2100 Bioanalyzer. • The contractor must deliver the kits within 30 days after award to USDA, SEA Plant Science Research, 840 Oval Dr., NC State University, 2122 Plant Sciences, Raleigh, NC 27606.
Attached Files:

Please visit the bid source via the “Link to Bid Source” button below for documentation.

Contact Information:

Kelly Bero

Kelly.Bero@usda.gov

Budget Estimate (AI):

$100,000 – $500,000

The budget for this contract is not explicitly stated, but based on the requirements, it can be estimated to be in the range of $100,000 to $500,000. This estimate is based on the quantity of kits required (at least 8640) and the complexity of the kits, which include unique DNA sequence barcodes and reagents for library preparation. The actual budget may vary depending on the contractor's pricing and the USDA's budget.

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