Sources Sought – RRBS Sequencing Services

The Department of Veterans Affairs is seeking sources for Reduced Representation Bisulfite Sequencing (RRBS) services to support DNA methylation profiling of post-mortem human choroid plexus tissue samples. The required services include sample handling and QC, bisulfite conversion, library preparation, Illumina paired-end sequencing, and raw data alignment, methylation calling, and genome browser track generation. The anticipated turnaround time is 6-8 weeks. • Sample handling and quality control (QC) assessment of submitted genomic DNA samples • Bisulfite conversion, library preparation, and QC assessment • Illumina paired-end sequencing at approximately 30 million read pairs per sample • Raw data alignment, single-base resolution methylation calling, and genome browser track generation • Deliverable formats: FASTQ (raw data), BAM (Bismark-aligned), BedGraph/Bismark coverage files (methylation calls), genome browser tracks