Q301--Zymo Epigenome Sequencing Services

The Department of Veterans Affairs intends to award a sole source contract to Zymo Research Corporation for Reduced Representation Bisulfite Sequencing (RRBS) services for DNA methylation profiling of 269 post-mortem human choroid plexus tissue samples. The services include sample handling and QC assessment, bisulfite conversion, library preparation and QC assessment, sequencing, and raw data alignment, methylation calling, and genome browser track generation. This requirement supports an ongoing VA research study requiring DNA methylation data from choroid plexus tissue that is directly comparable to an existing dataset previously generated by Zymo Research Corporation. • The contractor will provide RRBS services on 269 post-mortem human choroid plexus tissue samples. • The services include sample handling and QC assessment, bisulfite conversion, library preparation and QC assessment, sequencing, and raw data alignment, methylation calling, and genome browser track generation. • The requirement supports an ongoing VA research study requiring DNA methylation data from choroid plexus tissue. • Zymo Research Corporation is the only vendor that can generate this data using the same laboratory, personnel, and protocol as the existing dataset.